Protective effects of maternal methyl donor supplementation on adult offspring of high fat diet-fed dams |
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| Institution: | 1. Department of Biochemistry and Molecular Biology, Binzhou Medical College, Yantai, Shandong Province 264003, People''s Republic of China;2. Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People''s Republic of China;3. Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, Guangdong Province 523808, People''s Republic of China;4. Department of Biochemistry and Molecular Biology, Guizhou Medical University, Guiyang, Guizhou Province 550004, People''s Republic of China;5. Department of Medicine, College of Physicians and Surgeons, Columbia University New York, New York, NY 10027, USA;1. Department of Gynecology and Obstetrics, University Hospital, Faculty of Medicine of Ribeirão Preto, FMRP-USP, Ribeirão Preto, SP, Brazil;2. Department of Pediatrics, University Hospital, FMRP-USP, Ribeirão Preto, SP, Brazil;3. Department of Public Health, Federal University of Maranhão, MA, Brazil;4. Department of Gynecology and Obstetrics, University Hospital, FMRP-USP, Ribeirão Preto, SP, Brazil;1. Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Yanbian University, Ministry of Education, Yanji 133002, China;2. Department of Dermatology, Yanbian University Hospital, Yanji 133000, China;3. Department of Pathology, Yanbian University Medical College, Yanji 133002, China;4. Department of Pathology, Luhe Teaching Hospital of the Capital Medical University, Beijing 101100, China;1. Department of Obstetrics & Gynecology, St. Michael''s Hospital & University of Toronto, Toronto, ON, Canada;2. Department of Obstetrics and Gynecology, Assuta Ashdod University Hospital, Ashdod, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel;3. The Keenan Research Center for Biomedical Science of St. Michael''s Hospital, Toronto, Ontario, Canada;4. Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, Canada;5. Department of Medicine, University of Toronto, Toronto, Ontario, Canada;6. Division of Nutritional Sciences, College of Human Ecology, Cornell University, Ithaca, NY;7. Freelance statistics consultant, Toronto, Ontario, Canada;8. Research Institute, the Hospital for Sick Children;9. The Division of Gastroenterology, Department of Medicine, St. Michael''s Hospital, Toronto, Ontario, Canada |
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| Abstract: | Obesity has become a global public health problem associated with metabolic dysfunction and chronic disorders. It has been shown that the risk of obesity and the DNA methylation profiles of the offspring can be affected by maternal nutrition, such as high-fat diet (HFD) consumption. The aim of this study was to investigate whether metabolic dysregulation and physiological abnormalities in offspring caused by maternal HFD can be alleviated by the treatment of methyl donors during pregnancy and lactation of dams. Female C57BL/6 mice were assigned to specific groups and given different nutrients (control diet, Control + Met, HFD and HFD + Met) throughout gestation and lactation. Offspring of each group were weaned onto a control diet at 3 weeks of age. Physiological (weight gain and adipose composition) and metabolic (plasma biochemical analyses) outcomes were assessed in male and female adult offspring. Expression and DNA methylation profiles of obesogenic-related genes including PPAR γ, fatty acid synthase, leptin and adiponectin were also detected in visceral fat of offspring. The results showed that dietary supplementation with methyl donors can prevent the adverse effects of maternal HFD on offspring. Changes in the expression and DNA methylation of obesogenic-related genes indicated that epigenetic regulation may contribute to the effects of maternal dietary factors on offspring outcomes. |
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