| O139霍乱弧菌LPS基因在大肠杆菌中的克隆和表达 |
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| 引用本文： | 曲殿波,刘传暄,马清钧.O139霍乱弧菌LPS基因在大肠杆菌中的克隆和表达[J].生物工程学报,2000,16(6):699-702. |
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| 作者姓名： | 曲殿波 刘传暄 马清钧 |
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| 作者单位： | 军事医学科学院生物工程研究所,北京,100850 |
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| 基金项目： | 国家高技术研究发展与计划项目资助(102-07-03-02)。 |
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| 摘 要： | 利用粘粒载体pCOS5构建了国内分离的O139霍乱弧菌的基因组文库，并从文库中筛选获得可以表达O139霍乱弧菌脂多糖的重组克隆株E.coliJM109(pMG310)。重组粘粒pMG310经酶切分析，所克隆的外源DNA片段大小为37kb。实验证明：重组克隆株E.coliJM109(pMG310)所表达的脂多糖具有良好的免疫原性及反应原性。
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| 关 键 词： | 脂多糖，O139霍乱弧菌 |
| 文章编号： | 1000-3061-(2000)06-0699-04 |
| 修稿时间： | 2000-03-10 |
Cloning and Expression of Lipopolysaccharide Gene of Vibrio cholerae O139 in JM109 |
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| QU Dian-Bo,LIU Chuan-Xuan,MA Qing-Jun.Cloning and Expression of Lipopolysaccharide Gene of Vibrio cholerae O139 in JM109[J].Chinese Journal of Biotechnology,2000,16(6):699-702. |
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| Authors: | QU Dian-Bo LIU Chuan-Xuan MA Qing-Jun |
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| Institution: | QU Dian-Bo
,LIU Chuan-Xuan
,MA Qing-Jun
;(Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850) |
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| Abstract: | The gene cluster that determines the biosynthesis of the lipopolysaccharide(LPS) of V.cholerae O139 was cloned and expressed in E.coli JM109 by constructing genomic library of Vibrio cholerae O139. A colony that expressed LPS of Vibrio cholerae O139 was detected and designated E.coli JM109(pMG310). Restriction analysis of the recombinant cosmid pMG310 demonstrated that the DNA fragment was 52kb. The LPS gene of Vibrio cholerae O139 that was linked to Vector pCOS5 was about 37 kb. Immunochemical assays demonstrated that LPS expressed by E.coli JM109(pMG310) had the same immunogenicity and reactogenicity as LPS of Vibrio cholerae O139. |
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| Keywords: | LPS Vibrio cholerae O139 |
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