Ascorbate-glutathione metabolism during PEG-induced water deficit in <Emphasis Type="Italic">Trifolium repens</Emphasis> |
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Authors: | C Q Wang T Liu H J Xu |
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Institution: | 1.College of Life Sciences,Shandong University of Technology,Zibo, Shandong,China |
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Abstract: | In order to elucidate the response of the ascorbate-glutathione (ASC-GSH) cycle to drought stress, the activities of antioxidant
enzymes and the levels of molecules involved in the ASC-GSH metabolism were studied in Trifolium repens L. seedlings subjected to PEG-induced water deficit. Compared to the control, the contents of H2O2, thiobarbituric acid reactive substances (TBARS), ascorbate (ASC), dehydroascorbate (DHA), and glutathione disulfide (GSSG)
increased in PEG-treated seedlings, whereas the glutathione (GSH) content kept constant during the drought period. Further
more, the ASC/DHA and GSH/GSSG ratios decreased in the presence of PEG. Except for that of monodehydroascorbate reductase
(MDHAR), the activities of ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) were
up-regulated during water deficit, and the increases in APX and DHAR activities were much higher than those in GR activity.
These data indicate that fluctuations in the ASC-GSH metabolism resulted from PEG treatment may have a positive effect on
drought stress mitigation in T. repens. |
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Keywords: | |
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