Synthesis of and triplex formation in oligonucleotides containing 2′-deoxy-6-thioxanthosine |
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Authors: | Takeshi Inde Shuhei Nishizawa Yuusaku Hattori Takashi Kanamori Hideya Yuasa Kohji Seio Mitsuo Sekine Akihiro Ohkubo |
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Institution: | Department of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta, Midoriku, Yokohama 226-8501, Japan |
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Abstract: | This study aimed to synthesize triplex-forming oligonucleotides (TFOs) containing 2′-deoxy-6-thioxanthosine (s6X) and 2′-deoxy-6-thioguanosine (s6Gs) residues and examined their triplex-forming ability. Consecutive arrangement of s6X and s6Gs residues increased the triplex-forming ability of the oligonucleotides more than 50 times, compared with the unmodified TFOs. Moreover, the stability of triplex containing a mismatched pair was much lower than that of the full-matched triplex, though s6X could form a s6X-GC mismatched pair via tautomerization of s6X. The present results reveal excellent properties of modified TFOs containing s6Xs and s6Gs residues, which may be harnessed in gene therapy and DNA nanotechnology. |
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Keywords: | Triplex-forming oligonucleotide DNA nanotechnology Gene therapy Thioxanthosine |
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