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Application of Isoelectric Focusing to the Taxonomic Identification of Meloidogyne spp.
Authors:Edgar C Lawson  III  George E Carter  Jr  Stephen A Lewis
Abstract:Meloidogyne incognita, M. arenaria, M. hapla, and M. javanica were distinguishable from each other by isoelectric focusing (IEF) of nematode egg proteins. Proteins extracted from larvae and adults of Hoplolaimus columbus and from eggs of Heterodera glycines had distinctive profiles, also. Protein profiles from eggs, preparasitic larvae and egg-laying adults of M. incognita showed differences. It was necessary to compare samples run at the same time to ensure reliability.
Keywords:root-knot nematodes  biochemical taxonomy  isoelectric focusing  Meloidogyne spp    protein profiles
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