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CRISPR screens in physiologic medium reveal conditionally essential genes in human cells
Authors:Nicholas J. Rossiter  Kimberly S. Huggler  Charles H. Adelmann  Heather R. Keys  Ross W. Soens  David M. Sabatini  Jason R. Cantor
Affiliation:1. Morgridge Institute for Research, Madison, WI 53715, USA;2. Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA;3. Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA;4. Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;5. Koch Institute for Integrative Cancer Research, Cambridge, MA 02139, USA;6. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA;7. Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA;8. University of Wisconsin Carbone Cancer Center, Madison, WI 53705, USA
Abstract:
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  • Keywords:CRISPR  genetic screen  gene-nutrient interaction  conditional gene essentiality  HPLM  physiologic medium
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