Sinorhizobium meliloti plasmid pRm1132f replicates by a rolling-circle mechanism |
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| Authors: | Barran L R Ritchot N Bromfield E S |
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| Affiliation: | Soils and Crops Research and Development Centre, Agriculture and Agri-food Canada, Sainte-Foy, Quebec G1V 2J3, Canada. barranlr@em.agr.ca |
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| Abstract: | pRm1132f isolated from Sinorhizobium meliloti is a group III rolling-circle-replicating (RCR) plasmid. At least seven of eight open reading frames in the nucleotide sequence represented coding regions. The minimal replicon contained a rep gene and single- and double-stranded origins of replication. Detection of single-stranded plasmid DNA confirmed that pRm1132f replicated via an RCR mechanism. |
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