Fine Genetic Map of Mouse Chromosome 10 around the Polycystic Kidney Disease Gene,jcpk,and Ankyrin 3 |
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| Authors: | Elizabeth C Bryda Helen Ling Donald E Rathbun Margit Burmeister Lorraine Flaherty |
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| Institution: | aMolecular Genetics Program, Laboratory of Developmental Genetics, Wadsworth Center, New York State Department of Health, Albany, New York, 12208;bDepartment of Psychiatry, Department of Human Genetics, Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan, 48109 |
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| Abstract: | A chlorambucil (CHL)-induced mutation of thejcpk(juvenile congenital polycystic kidney disease) gene causes a severe early onset polycystic kidney disease. In an intercross involvingMus musculus castaneus, jcpkwas precisely mapped 0.2 cM distal toD10Mit115and 0.8 cM proximal toD10Mit173.In addition, five genes,Cdc2a, Col6a1, Col6a2, Bcr,andAnk3were mapped in both thisjcpkintercross and a (BALB/c × CAST/Ei)F1× BALB/c backcross. All five genes were eliminated as possible candidates forjcpkbased on the mapping data. Thejcpkintercross allowed the orientation of theAnk3gene relative to the centromere to be determined.D10Mit115, D10Mit173, D10Mit199,andD10Mit200were separated genetically in this cross. The order and genetic distances of all markers and gene loci mapped in thejcpkintercross were consistent with those derived from the BALB/c backcross, indicating that the CHL-induced lesion has not generated any gross chromosomal abnormalities detectable in these studies. |
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