Effects of concentrated supernatants recovered from Lactobacillus plantarum on Escherichia coli growth and on the viability of a human promyelocytic cell line |
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Authors: | E. Puertollano,M.A. Puertollano,L. Cruz-Chamorro,G.Á . de Cienfuegos,A. Ruiz-Bravo, M.A. de Pablo |
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Affiliation: | Unit of Microbiology, Department of Health Sciences, Faculty of Experimental Sciences, University of Jaén, Jaén, Spain; Department of Metabolism and Nutrition, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain; Department of Microbiology, Faculty of Pharmacy, University of Granada, Granada, Spain |
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Abstract: | Aims: The ability of concentrated supernatants from Lactobacillus plantarum to produce a disruption of plasma membrane in eukaryotic and prokaryotic cells has been examined. Methods and Results: A strain of Lact. plantarum (tolerant to acid and bile salts and resistant to several antibiotics) was used. It inhibited the growth of pathogenic Escherichia coli and L. monocytogenes . Supernatants from Lact. plantarum were concentrated by centrifugation. Either E. coli or HL-60 cells (a human promyelocytic cell line) were treated in the presence of the concentrated supernatants. The effect of concentrated supernatants from Lact. plantarum on E. coli growth demonstrated a bacteriostatic activity and a loss of cell viability measured by sytox green staining. Concentrated supernatants were capable of disturbing plasma membrane in E. coli and of promoting a cytotoxic and lyctic action on HL-60 cells and on human erythrocytes, respectively. Conclusions: These results suggest that Lact. plantarum release an effective compound responsible for an important effect in the disruption of E. coli plasma membrane and for a cytototoxic activity on promyelocytic leukaemia cells. Significance and Impact of the Study: This is the first in vitro study about the antimicrobial and biological activities of concentrated supernatants from Lact. plantarum . |
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Keywords: | concentrated supernantants HL-60 cells lactic acid bacteria Lactobacillus plantarum probiotics |
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