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Estrogen productivity of endometrium and endometrial cancer tissue; influence of aromatase on proliferation of endometrial cancer cells |
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| Authors: | Takara Yamamoto Jo Kitawaki Mamoru Urabe Hideo Honjo Takaya Tamura Toshifumi Noguchi Hiroji Okada Hiroshi Sasaki Akio Tada Yoshiteru Terashima Junji Nakamura Makoto Yoshihama |
| Institution: | 1 Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kamikyo-ku, Kyoto 602, Japan 2 Department of Obstetrics and Gynecology, Jikei University School of Medicine, Minato-ku, Tokyo 105, Japan 3 Research Institute, Life Science, Snow Brand Milk Prod. Co., Tochigi 329-05, Japan |
| Abstract: | Aromatase, estrone (E1) sulfatase and E1 sulfotransferase activities were examined in endometrium and endometrial cancer tissue preparations. Aromatase and E1 sulfatase activities in endometrial cancer tissues were found to be significantly higher than in normal endometrial tissues. However, E1 sulfotransferase activity did not differ between benign and malignant tissue. We also examined the effect of testosterone (T) on aromatase activity and tritiated thymidine uptake (DNA synthesis) in various cultured cervical or corpus endometrial cancer cell lines (OMC-4, HHUA, Ishikawa, HEC-59). The results demonstrated that only the HEC-59 cell line had high aromatase activity and increased its DNA synthesis in response to T. This increase of DNA synthesis by T was not suppressed by simultaneous addition of cyproterone acetate, but was by tamoxifen. These data suggest that in situ estrogen production in endometrial cancer tissue is biologically important and that aromatase in cancer cells may contribute partially to cell proliferation if androgen substrate is provided. |
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