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Effect of hydralazine on myocardial plasma membrane fatty acid binding protein (PM-FABP) during diabetes mellitus |
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| Authors: | Clayton E. Heyliger David M. Powell Kenneth A. Skau |
| Affiliation: | (1) Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, 351 Tache Avanue, R2H 2A6 Winnipeg, MB, Canada;(2) Department of Physiology, University of Manitoba, Canada;(3) Department of Biochemistry and Molecular Biology, University of Manitoba, Canada;(4) Present address: CHUL Research Centre, 2705 Boulevard Laurier, Ste-Foy, Quebec;(5) Present address: Research Centre, Montreal Heart Institute, 5000 east, Belanger Street, Montreal, Quebec, Canada |
| Abstract: | Irregularities in K+ currents form the basis of several cardiovascular dysfunctions, among which are arrhythmias and vasospasms. The developmental regulation of voltage-gated K+ channels, however, has been difficult to study. A novel approach was therefore employed to examine these channels in muscle tissue. Primers for a PCR-based analysis were designed using published nucleic acid sequences for voltage-gated K+ channels. Final selection of the primer pairs was based on the homology present in the S4 and H5 transmembrane domains. A specific band was amplified with these primers using RNA isolated from both rat A10 vascular smooth muscle cells and rat heart tissue. |
| Keywords: | diabetes mellitus cardiomyopathy hydralazine cardiomyocytes fatty acids fluorescence trans-parinaric acid cis-parinaric acid plasma membrane fatty acid binding protein |
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