Absorbance and fluorescence properties of protochlorophyllide in etiolated bean leaves |
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Authors: | M L Gassman |
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Institution: | Dept. of Biological Sciences University of Illinois at Chicago Circle Chicago, Illinois 60680 USA |
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Abstract: | Primary leaves of 7-to-9 day-old etiolated bean seedlings contain a species of protochlorophyllide which is not transformed to chlorophyllide by light; this pigment species exhibits an absorption peak at 631nm at ?196° and a fluorescence emission peak at 639nm at room temperature. Heat-treatment of etiolated leaves converts the phototransformable protochlorophyllide holochrome to a pigment species with absorption and fluorescence peaks identical to those of endogenous nontransformable protochlorophyllide. Administration of δ-amino-levulinic acid to etiolated leaves causes the synthesis of non-transformable protochlorophyllide with an absorption peak also at 631nm at ?196° but with a fluorescence emission peak at 643nm at room temperature. Heat-treatment of such leaves does not affect the position of these bands. The results indicate that protochlorophyllide which is derived from exogenous δ-amino-levulinic acid is in a physically different state from other forms of protochlorophyllide in the leaf. |
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