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Flavonoids of <Emphasis Type="Italic">Rosa roxburghii</Emphasis> Tratt offers protection against radiation induced apoptosis and inflammation in mouse thymus
Authors:Sai-Juan Xu  Fan Zhang  Li-Juan Wang  Ming-Hua Hao  Xian-jun Yang  Na-na Li  Hong-long Ji  Ping Xu
Institution:1.Department of Pharmacy,Xinxiang Medical University,Xinxiang,China;2.International Joint Research Laboratory for Recombiant Pharmaceutical Protein Expression System of Henan,Xinxiang Medical University,Xinxiang,China;3.Henan Key Laboratory of Medical Tissue Regeneration,Xinxiang Medical University,Xinxiang,China;4.Department of Cellular and Molecular Biology,University of Texas Health Northeast,Tyler,USA;5.Institute of Lung and Molecular Therapy,Xinxiang Medical University,Xinxiang,China
Abstract:The present study evaluated the protective effect of the natural compound flavonoids of Rosa roxburghii Tratt (FRT) against γ-radiation-induced apoptosis and inflammation in mouse thymus cells in vivo and in vitro. Thymus cells and mice were exposed to 60Co γ-ray at a dose of 6 Gy. The radiation treatment induced significant cell apoptosis and inflammation. Radiation increased the expressions of cleaved caspase 3/8–10, AIF, and PARP-1, and FRT could mitigate their activation and inhibit subsequent apoptosis in the thymus both in vitro or in vivo. Irradiation increased the mRNA expression of ICAM-1/VCAM-1, IL-1α/IL-6 and TNF-α/NF-κB. Our results also indicated that FRT alleviated gene expression of some inflammatory factors such as ICAM-1/VCAM-1, TNF-α/NF-κB, but not IL-1α/IL-6. Irradiation increased the protein expression levels of ICAM-1/VCAM-1, IL-1α/IL-6 and TNF-α/NF-Κb, and our results also indicated that FRT alleviated protein level expression of certain inflammatory factors such as ICAM-1, IL-1α/IL-6, TNF-α/NF-κB, but not VCAM-1. Our results suggested that FRT enhanced radioprotection at least partially by regulating caspase 3/8–10, AIF, and PARP-1 to reduce apoptosis and by regulating ICAM-1, IL-1α/IL-6, TNF-α/NF-κB to reduce inflammation.
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