高对映选择性环氧化物水解酶产生菌的筛选及特性研究

从土壤中分离的芽孢杆菌Bacillus megaterium ECU1001所产五氧化物水解酶能高对映选择性水解缩水甘油苯基醚（对映选择率E值可达47.8），当转化率为55.9%时，剩余的（S）－缩水甘油苯基醚的光学纯度（对映体过量值，ee）可达99.5%；当底物浓度提高到60mmol/L时，光学纯（S）－缩水基油苯基醚的收率达到25.6%。

ISOLATION AND CHARACTERIZATION OF AN EPOXIDE HYDROLASE PRODUCER FOR ENANTIOSELEC TIVE HYDROLYSIS OF (R, S )-PHENYL GLYCIDYL ETHER

A bacterial strain (ECU1001) was isolated from soil and identified to be Bacillus megaterium.The strain shows an activity of epoxide hydrolase that catalyzes the asymmetric hydrolysis of phenyl glycidyl ether,yielding (S)-epoxide and (R)-diol with high enantioselectivity (E=47.8).When the racemic substrate was enzymaticlly hydrolyzed to 55.9% conversion,the enantiomeric excess (ee) of recovered (S)-epoxide was 99.5%.The yield of (S)-phenyl glycidyl ether could reach 25.6% when concentration of the substrate was increased to 60 mmol/L.