Location of rRNA genes in three inbred strains of rat and suppression of rat rRNA activity in rat-human somatic cell hybrids |
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| Authors: | R. Tantravahi D. A. Miller G. D''Ancona C. M. Croce O. J. Miller |
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| Affiliation: | 1. Department of Human Genetics and Development, Columbia University, New York, NY 10032, USA;2. The Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104, USA;3. Department of Obstetrics and Gynecology, Columbia University, New York, NY 10032, USA |
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| Abstract: | Nucleolus organizer regions (NORs) of rat chromosomes were stained by the Ag-AS method. The Ag-NORs were found on chromosomes 3, 11 and 12 in the ACI, Wistar Brown and Wistar Lewis inbred strains of rat. The size of the Ag-NOR on each pair of chromosomes varied from strain to strain. Rat-human somatic hybrid cells that retained human and lost some of the rat chromosomes had no Ag-NOR on rat chromosomes 3, 11 or 12. Since NORs can be Ag-stained only if their 18 + 28S rRNA genes are active, the activity of the rat rRNA genes must have been suppressed in the hybrid cells. |
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