PEP Carboxykinase Exchange Reaction in Photosynthetic Bacteria |
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| Authors: | T G Cooper and C R Benedict |
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| Institution: | Chemistry Department, Wayne State University, Detroit, Michigan |
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| Abstract: | This paper describes some new characteristics of the phosphoenolpyruvate carboxykinase CO(2)-oxaloacetate exchange reaction in purified preparations of Rhodospirillum rubrum. The enzymatic activity has been purified 169-fold. Nucleotide diphosphates substitute for nucleotide triphosphates in the exchange reaction. Nucleotide diphosphates will not support the synthesis of phosphoenolpyruvate from oxaloacetate. This reaction differs significantly from the CO(2)-oxaloacetate exchange reaction in higher plants and animals. |
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