Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress |
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Authors: | Zallocchi Marisa Matković Laura Damasco María C |
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Affiliation: | Dpto. Química Biológica, Facultad de Ciencias Exactas y Naturales (UBA), Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, Argentina. mzallo@qb.fcen.uba.ar |
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Abstract: | This work studied the effect of stresses produced by simulated gavage or gavage with 200 mmol/L HCl two hours before adrenal extraction, on the activities of the 11beta-hydroxysteroid dehydrogenase 1 and 11beta-hydroxysteroid dehydrogenase 2 isoforms present in the rat adrenal gland. These activities were determined on immediately prepared adrenal microsomes following incubations with 3H-corticosterone and NAD+ or NADP+. 11-dehydrocorticosterone was measured as an end-product by TLC, and controls were adrenal microsomes from rats kept under basal (unstressed) conditions. 11beta-hydroxysteroid dehydrogenase 1 activity, but not 11beta-hydroxysteroid dehydrogenase 2 activity, was increased under both stress-conditions. Homeostatically, the stimulation of 11beta-hydroxysteroid dehydrogenase 1 activity would increase the supply of glucocorticoids. These, in turn, would activate the enzyme phenylethanolamine N-methyl transferase, thereby improving the synthesis of epinephrine as part of the stress-response. |
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