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The Expression of a Cytosolic Cyclophilin Promoter from Periwinkle in Transgenic Tobacco Plants
Authors:A-M Droual  J Creche  F Andreu  J-C Chenieux  M Rideau  S Hamdi
Institution:(1) Cropdesign NV, Technologiepark 3, B-9052 Zwijnaarde, Belgium;(2) Faculty of Pharmacy, EA2106, 31 Avenue Monge, F-37200 Tours, France;(3) Faculty of Sciences, UMR 619, BCMDP, Avenue des Facultés, F-33405 Talence, France
Abstract:The cloning of a 465 bp fragment from the 5prime-flanking region of the gene encoding a cytosolic cyclophilin from periwinkle was achieved through inverse polymerase chain reaction. The DNA fragment was fused to a gusA-intron marker then introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. Histochemical analysis of the transgenic shoot cultures demonstrated that the construct was able to drive GUS expression in stomata guard cells, but not in mesophyll cells when shoots were still attached to the callus from which they were initiated. In separated transgenic shoots and in seedlings, GUS was expressed in external and internal phloem and root hairs, respectively. GUS activity in transgenic tobacco seedlings was also investigated by fluorimetric assays. Treatments with NaCl or ABA decreased promoter activity whereas treatment with yeast extracts increased it.
Keywords:Catharanthus roseus  GUS expression  promoter analysis  stress wounding
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