| 镉胁迫对拟南芥MLH1基因启动子甲基化的影响 |
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| 引用本文： | 单存海,钟鸣,刘宛,郭志富,牛建双,周启星. 镉胁迫对拟南芥MLH1基因启动子甲基化的影响[J]. 植物生理学通讯, 2011, 0(3): 298-304 |
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| 作者姓名： | 单存海 钟鸣 刘宛 郭志富 牛建双 周启星 |
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| 作者单位： | 沈阳农业大学生物科学技术学院;中国科学院沈阳应用生态研究所;南开大学环境科学与工程学院; |
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| 基金项目： | 国家自然科学基金(20977095,31070448); 中国科学院陆地生态过程重点实验室开放课题 |
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| 摘 要： | 采用重亚硫酸盐测序和结合重亚硫酸盐限制性分析技术研究了镉（Cd）胁迫对拟南芥幼苗错配修复基因MutL—homologue 1（MLH1）启动子甲基化的影响，并结合幼苗的形态和生理指标，筛选Cd胁迫敏感的生物标记物。结果表明：不同浓度（0、0．125、0．25、1．0、3．0mg·L^-1）Cd处理3d对幼苗叶片数、地上部鲜重、地上部叶绿素含量影响不大，根长和地上部可溶性蛋白质含量随Cd浓度的增加较对照显著降低（P〈0．05），幼苗MLH1启动子甲基化水平随Cd浓度的增加较对照明显上升。以上结果表明，基因舰驯启动子甲基化的变化可作为检测Cd污染对植物遗传毒性效应潜在的、有用的生物标记物。
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| 关 键 词： | Cd MLH1 生物标记物 启动子 甲基化 |
Effects of Cadmium Stress on MLH1 Promoter Methylation in Arabidopsis thaliana |
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| SHAN Cun-Hai,ZHONG Ming,LIU Wan,GUO Zhi-Fu,NIU Jian-Shuang,ZHOU Qi-Xing, College of Biological Science , Technology,Shenyang Agricultural University,Shenyang ,China. Effects of Cadmium Stress on MLH1 Promoter Methylation in Arabidopsis thaliana[J]. Plant Physiology Communications, 2011, 0(3): 298-304 |
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| Authors: | SHAN Cun-Hai ZHONG Ming LIU Wan GUO Zhi-Fu NIU Jian-Shuang ZHOU Qi-Xing College of Biological Science Technology Shenyang Agricultural University Shenyang China |
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| Affiliation: | SHAN Cun-Hai1,ZHONG Ming1,LIU Wan2,GUO Zhi-Fu1,NIU Jian-Shuang1,ZHOU Qi-Xing2,3 1College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110866,China,2Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,3College of Environmental Science and Engineering,Nankai University,Tianjin 300071 |
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| Abstract: | Using bisulfite DNA sequencing and combined bisulfite restriction analysis technique, effects of cadmium （Cd） stress on methylation of MutL-homologue 1 （MLH1） promoter of mismatch repair gene of Arabidopsis thaliana seedlings were studied and linked to morphological and physiological indices, aimed to choose the biomarkers sensitive to Cd stress. The results indicated that under Cd （0, 0.125, 0.25, 1.0, 3.0 mg.L^-1） stress for 3 days, no significant changes were observed in leaf number, fresh mass and chlorophyll content of aerial parts. Compared with the control, root length and total soluble protein content of aerial parts decreased significantly with increasing Cd concentration （P〈0.05）, MLH1 promoter methylation of seedlings was generally increased with increasing Cd concentration. The above results suggested that specific methylation of MLH1 promoter could serve as the potential and useful biomarker for the detection ofgenotoxic effects of Cd pollution on plants. |
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| Keywords: | Cd MLH1 biomaker promoter methylation |
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