| 二色补血草LbDREB基因在酵母中的抗逆性分析 |
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| 引用本文： | 王留强,班巧英,贺琳,王玉成. 二色补血草LbDREB基因在酵母中的抗逆性分析[J]. 植物生理学通讯, 2011, 0(4): 347-350 |
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| 作者姓名： | 王留强 班巧英 贺琳 王玉成 |
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| 作者单位： | 东北林业大学林木遗传育种与生物技术教育部重点实验室; |
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| 基金项目： | 国家自然科学基金(30972387); 教育部新世纪优秀人才计划(NCET-08-0751) |
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| 摘 要： | 利用酵母表达系统研究了二色补血草的DREB基因（LbDREB）对不同胁迫的抗性。将LbDREB构建到酵母表达载体pYES2中,转化到酿酒酵母INVSc1菌株中,并以转空pYES2质粒的酵母INVSc1（pYES2）作为对照,通过比较两种酵母在不同胁迫下的存活率来研究LbDREB基因对NaCl、KH_2PO_4、Na_2CO_3、NaHCO_3、低温、干旱、CuSO_4和CdCl_2胁迫的抗性。结果表明,LbDREB转化的酵母在各种胁迫下的存活率均明显高于转空pYES2的对照酵母,说明LbDREB基因除了具有传统认为的抗旱、耐盐、抗寒的作用外,还具有抗KH_2PO_4、Na_2CO_3、NaHCO_3、CuSO_4和CdCl_2等胁迫的能力。
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| 关 键 词： | 二色补血草 DREB基因 抗逆 酵母表达 |
Stress Tolerance Analysis of LbDREB Gene from Limonium bicolor Kuntze in Yeast |
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| WANG Liu-Qiang,BAN Qiao-Ying,HE Lin,WANG Yu-Cheng Key Laboratory of Forest Tree Genetic Improvement , Biotechnology,Ministry of Education,Northeast Forestry University,Harbin ,China. Stress Tolerance Analysis of LbDREB Gene from Limonium bicolor Kuntze in Yeast[J]. Plant Physiology Communications, 2011, 0(4): 347-350 |
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| Authors: | WANG Liu-Qiang BAN Qiao-Ying HE Lin WANG Yu-Cheng Key Laboratory of Forest Tree Genetic Improvement Biotechnology Ministry of Education Northeast Forestry University Harbin China |
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| Affiliation: | WANG Liu-Qiang,BAN Qiao-Ying,HE Lin,WANG Yu-Cheng~* Key Laboratory of Forest Tree Genetic Improvement and Biotechnology,Ministry of Education,Northeast Forestry University,Harbin 150040,China |
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| Abstract: | To investigate the stress tolerance of LbDREB,the recombinant plasmid pYES2-LbDREB was constructed by inserting the LbDREB gene into the yeast expression vector pYES2.The recombinant plasmid pYES2-LbDREB was transformed into yeast Saccharomyces cerevisiae INVSc1,and the INVSc1 strain transformed with empty pYES2 was used as the control.The recombinant yeast INVSc1 harboring LbDREB (pYES2-LbDREB) and the control INVSc1 harboring empty pYES2 were respectively treated with NaCl,KH_(2-) PO_4,Na_2CO_3,NaHCO_3,fr... |
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| Keywords: | Limonium bicolor DREB gene stress tolerance yeast expression |
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