| 伊贝母愈伤组织细胞内微管网络的免疫学观察 |
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| 引用本文： | 徐子勤 林忠平. 伊贝母愈伤组织细胞内微管网络的免疫学观察[J]. 西北植物学报, 1994, 14(3): 169-173 |
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| 作者姓名： | 徐子勤 林忠平 |
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| 作者单位： | 中国科学院植物研究所，兰州大学生物系 |
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| 摘 要： | 通过解聚－聚合循环过程纯化鸡脑蛋白，免疫家兔得到抗血清。采用免疫酶标技术显示出伊贝母愈伤组织细胞内的微管网络。用１％Ｔ ｒｉｔｏｎＸ－１００洗去细胞内其它成分，用８％ＮａＮ３消除内源过氧化物酶活性。实验结果提出了一种显示植物细胞内微管网络的方法。
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| 关 键 词： | 微管 微管蛋白 愈伤组织 伊贝母 |
IMMUNOLOGICAL OBSERVATION OF MICROTUBULE NETWORK EXISTED IN FRITILLARY(FRITILLARIA PALLIDIFLORA SCHRENK)CALLUS CELL |
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| Xu Ziqin,Lin Zhongpin and Stin Jingsan. IMMUNOLOGICAL OBSERVATION OF MICROTUBULE NETWORK EXISTED IN FRITILLARY(FRITILLARIA PALLIDIFLORA SCHRENK)CALLUS CELL[J]. Acta Botanica Boreali-Occidentalia Sinica, 1994, 14(3): 169-173 |
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| Authors: | Xu Ziqin Lin Zhongpin Stin Jingsan |
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| Abstract: | The pure tubulin of chicken brain was obtained through assembly-disassembly process,andwas used to inject three rabbits to get its antibody. The microtubule network of fritillary callus cell was showed through immunoenzymological technique. From this result,it propose a techniqucof showing plant cell microtubule network.The other components was washed away with 1%Triton X-100,and the internal peroxidase activity was eliminated with 8%NaN_3. |
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| Keywords: | microtubule tubulin assembly-disassembly Immunoenzymological technique |
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