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Allelic state of the molecular marker for golden nematode (Globodera rostochiensis) resistance gene H1 among Ukrainian and world potato (Solanum tuberosum ssp. tuberosum) cultivars |
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| Authors: | A V Karelov L A Pylypenko N O Kozub R O Bondus O I Borzykh I A Sozinov Ya B Blume A A Sozinov |
| Institution: | 1. Institute of Plant Protection, National Academy of Agrarian Sciences of Ukraine, Kyiv, Ukraine 2. State Institution “Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine”, Kyiv, Ukraine 3. Ustimivka Experimental Station of Plant Growing, Poltava oblast, Ukraine |
| Abstract: | The purpose of this study was to determine the allelic state of the resistance gene H1 against the Ro1 and Ro4 pathotypes of the golden potato cyst nematode (Globodera rostochiensis) among Ukrainian and world potato (Solanum tuberosum ssp. tuberosum) cultivars. The allelic state of the TG689 marker was determined by PCR with DNA samples isolated from potato tubers and primers, one pair of which flanked the allele-specific region and the other one was used to control the DNA quality. Among 77 potato cultivars analyzed, the allele of the marker associated with the H1-type resistance was found in 74% of Ukrainian and 90% of foreign cultivars, although some of them proved to be susceptible to the potato cyst nematode in the field. The obtained data confirm the presence of H1 resistance against golden nematode pathotypes Ro1 and Ro4 among Ukrainian potato cultivars and the efficiency of the used marker within the accuracy that has been declared by its authors. |
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