<Emphasis Type="Italic">In vitro</Emphasis> propagation of the genus <Emphasis Type="Italic">Clivia</Emphasis> |
| |
Authors: | Yidong Ran Sandra Simpson |
| |
Institution: | (1) Multiflora Laboratories Limited, P. O. Box 9516, Newmarket, Auckland, New Zealand;(2) Gene Technology Group, The Horticulture and Food Research Institute of New Zealand Limited, P.O. Box 92 169, Newmarket, Auckland, New Zealand |
| |
Abstract: | We have developed a protocol for the in vitro propagation of the genus Clivia. Shoots were regenerated when fragments of the peduncle-pedicel junction (PP junction) from young inflorescences were used as explants. The optimal media for PP junction were Murashige and Skoog (MS)-based medium containing 10 M of 6-benzyladenine (BA) and 10 M of 2,4-dichlorophenoxyacetic acid (2,4-D) or MS supplemented with 5 M BA, 10 M -naphthaleneacetic acid (NAA), 250 mg l-1 glutamine and 500 mg l–1 casein hydrolysate and their usage depended on the breeding lines. Multiplication from initiations and in vitro seedlings was the best when the explants were cut longitudinally through the meristem and placed on MS plus 44 M BA. Plantlets were transferred on to hormone -free MS medium with charcoal for rooting. |
| |
Keywords: | Clivia micropropagation peduncle– pedicel junction |
本文献已被 SpringerLink 等数据库收录! |
|