MicroRNA expression profiling of the fifth-instar posterior silk gland of Bombyx mori |
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Authors: | Jisheng Li Yimei Cai Lupeng Ye Shaohua Wang Jiaqian Che Zhengying You Jun Yu Boxiong Zhong |
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Affiliation: | .College of Animal Sciences, Zhejiang University, Hangzhou, Hangzhou, 310058 P.R. China ;.Institute of Sericulture, Chengde Medical University, Chengde, 067000 P.R. China ;.Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029 China |
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Abstract: | BackgroundThe growth and development of the posterior silk gland and the biosynthesis of the silk core protein at the fifth larval instar stage of Bombyx mori are of paramount importance for silk production.ResultsHere, aided by next-generation sequencing and microarry assay, we profile 1,229 microRNAs (miRNAs), including 728 novel miRNAs and 110 miRNA/miRNA* duplexes, of the posterior silk gland at the fifth larval instar. Target gene prediction yields 14,222 unique target genes from 1,195 miRNAs. Functional categorization classifies the targets into complex pathways that include both cellular and metabolic processes, especially protein synthesis and processing.ConclusionThe enrichment of target genes in the ribosome-related pathway indicates that miRNAs may directly regulate translation. Our findings pave a way for further functional elucidation of these miRNAs and their targets in silk production.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-410) contains supplementary material, which is available to authorized users. |
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Keywords: | MicroRNA Silkworm Posterior silk gland Target gene |
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