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The Mn4Ca photosynthetic water-oxidation catalyst studied by simultaneous X-ray spectroscopy and crystallography using an X-ray free-electron laser |
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| Authors: | Rosalie Tran Jan Kern Johan Hattne Sergey Koroidov Julia Hellmich Roberto Alonso-Mori Nicholas K. Sauter Uwe Bergmann Johannes Messinger Athina Zouni Junko Yano Vittal K. Yachandra |
| Affiliation: | 1.Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA;2.LCLS, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA;3.Institutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, Umeå, Sweden;4.Institut für Biologie, Humboldt-Universität Berlin, Berlin 10099, Germany |
| Abstract: | The structure of photosystem II and the catalytic intermediate states of the Mn4CaO5 cluster involved in water oxidation have been studied intensively over the past several years. An understanding of the sequential chemistry of light absorption and the mechanism of water oxidation, however, requires a new approach beyond the conventional steady-state crystallography and X-ray spectroscopy at cryogenic temperatures. In this report, we present the preliminary progress using an X-ray free-electron laser to determine simultaneously the light-induced protein dynamics via crystallography and the local chemistry that occurs at the catalytic centre using X-ray spectroscopy under functional conditions at room temperature. |
| Keywords: | manganese oxygen-evolving complex photosystem II X-ray crystallography X-ray emission spectroscopy X-ray free-electron laser |