Protein-Protein Interactions Leading to Recruitment of the Host DNA Sliding Clamp by the Hyperthermophilic Sulfolobus islandicus Rod-Shaped Virus 2 |
| |
| Authors: | Andrew F Gardner Stephen D Bell Malcolm F White David Prangishvili Mart Krupovic |
| |
| Institution: | aNew England Biolabs, Ipswich, Massachusetts, USA;bMolecular and Cellular Biochemistry Department and Biology Department, Indiana University, Bloomington, Indiana, USA;cBiomedical Sciences Research Complex, University of St Andrews, Fife, United Kingdom;dInstitut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, Département de Microbiologie, Paris, France |
| |
| Abstract: | Viruses infecting hyperthermophilic archaea typically do not encode DNA polymerases, raising questions regarding their genome replication. Here, using a yeast two-hybrid approach, we have assessed interactions between proteins of Sulfolobus islandicus rod-shaped virus 2 (SIRV2) and the host-encoded proliferating cell nuclear antigen (PCNA), a key DNA replication protein in archaea. Five SIRV2 proteins were found to interact with PCNA, providing insights into the recruitment of host replisome for viral DNA replication. |
| |
| Keywords: | |
|
|
