High-Density 3D Single Molecular Analysis Based on Compressed Sensing |
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| Authors: | Lusheng Gu Yi Sheng Yan Chen Hao Chang Yongdeng Zhang Pingping Lv Wei Ji Tao Xu |
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| Affiliation: | †College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, P. R. China;‡National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P. R. China;§School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, P. R. China |
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| Abstract: | Single molecule fitting-based superresolution microscopy achieves sub-diffraction-limit image resolution but suffers from a need for long acquisition times to gather enough molecules. Several methods have recently been developed that analyze high molecule density images but most are only applicable to two dimensions. In this study, we implemented a high-density superresolution localization algorithm based on compressed sensing and a biplane approach that provides three-dimensional information about molecules, achieving super-resolution imaging at higher molecule densities than those achieved using the conventional single molecule fitting method. |
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