Somatic embryogenesis of Agave victoria-reginae Moore |
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Authors: | Benjamin Rodríguez-Garay Antonia Gutiérrez-Mora Beatríz Acosta-Duefias |
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Affiliation: | (1) Departamento de Cultivo de Tejidos Vegetales, División de Biotecnología, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Avenida Normalistas 800, 44270 Guadalajara, Jalisco, México;(2) Present address: Instituto de Ciencias, A.C., Av. Manuel Avila Camacho No. 2935, 45160 Zapopan, Jalisco, México |
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Abstract: | Plant regeneration through direct somatic embryogenesis of leaf blade explants from in vitro propagated plants of Agave victoria-reginae Moore, is described. Somatic embryogenesis was evident in a 6-week period on agarsolidified MS medium supplemented with L2 vitamins and 2,4-dichlorophenoxyacetic acid (1,4 µM), and germination of somatic embryos was achieved after 8 weeks on half-strength MS medium and 4 weeks on half-strength SH medium, both lacking growth regulators. Hyperhydricity of somatic embryos and plantlets was reduced by the use of vented culture vessel lids during the last 4 weeks on SH medium. Shoot proliferation was obtained, and hyperhydricity was eliminated on a modified MS medium (with NH4NO3 reduced to 5 mN) supplemented with kinetin (4.6 µM) and 1-naphthaleneacetic acid (1.6 µM) and the use of vented culture vessel lids.Abbreviations 2,4-d 2,4-dichlorophenoxyacetic acid - NAA 1-naphthaleneacetic acid - MS Murashige and Skoog - LOG-1 MS modified medium by Castro-Concha et al. (1990) - L2 Phillips and Collins (1979) vitamins - SH Schenk and Hildebrandt |
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Keywords: | Agavaceae hyperhydricity threatened species tissue eulture |
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