A quantitative assay for tyrosine sulfation and tyrosine phosphorylation in peptides. |
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| Authors: | H Blode T Heinrich H Diringer |
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| Institution: | Robert Koch-Institut des Bundesgesundheitsamtes, Berlin. |
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| Abstract: | A method was developed to measure sulfation and phosphorylation of tyrosine in proteins after alkaline hydrolysis, ion-exchange chromatography, reaction with 3H]dinitrofluorobenzene and subsequent thin-layer chromatography. The method allows the detection of 10-20 pmol of modified tyrosine and was applied to determine the content of tyrosine-phosphate and -sulfate in fibrinogens, thyroglobulin, alpha-casein, cytochrome c and glyceraldehyde dehydrogenase. |
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