| 花椒cpDNA trnL-F间隔区序列的特征及其在混淆品鉴别中的应用 |
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| 引用本文： | 沈洁,丁小余,张卫明,保曙琳,常俊,唐凤.花椒cpDNA trnL-F间隔区序列的特征及其在混淆品鉴别中的应用[J].中国野生植物资源,2004,23(3):29-32. |
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| 作者姓名： | 沈洁 丁小余 张卫明 保曙琳 常俊 唐凤 |
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| 作者单位： | 1. 南京师范大学生命科学学院,江苏,南京,210097
2. 南京野生植物综合利用研究院,江苏,南京,210042 |
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| 基金项目： | 国家农业领域科技攻关项目 |
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| 摘 要： | 目的:通过分析花椒cpDNA trnL-F间隔区序列特点,探讨该序列在花椒与其混淆品鉴别中的意义.方法:对花椒及其混淆品进行cpDNA trnL-F间隔区序列比较研究.结果:花椒cpDNA trnL-F间隔区序列为349bp、AT百分比为62.1%.尽管花椒的果实在外部形态,物理特性上与混淆品差异较小,但在cpDNA trnL-F间隔区序列上却存在着显著而稳定的差异.该序列在属以下的分类阶元中同源性极高,可达100%.结论:根据cpDNA trnL -F间隔区碱基序列的差异,能有效地鉴别花椒及其属以上分类阶元的混淆品.
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| 关 键 词： | 花椒 cpDNA trnL-F间隔区序列 混淆品 鉴别 |
| 修稿时间： | 2003年7月8日 |
Characteristics of cpDNA trnL- F Sequences of Zanthexylum bungeanum Maxim.and Authentication from Its Forgeries by Those Sequences |
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| Shen Jie ,Ding Xiaoyu ,Zhang Weiming ,Bao Shulin ,Chang Jun ,Tang Feng.Characteristics of cpDNA trnL- F Sequences of Zanthexylum bungeanum Maxim.and Authentication from Its Forgeries by Those Sequences[J].Chinese Wild Plant Resources,2004,23(3):29-32. |
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| Authors: | Shen Jie Ding Xiaoyu Zhang Weiming Bao Shulin Chang Jun Tang Feng |
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| Institution: | Shen Jie 1,Ding Xiaoyu 1,Zhang Weiming 2,Bao Shulin 1,Chang Jun 1,Tang Feng 1 |
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| Abstract: | Aim:To study the authentication of Zanthexylum bungeanum Maxim. from the fake ones by means of analyzing their cpDNA trnL-F sequences. Methods:compare the cpDNA trnL-F regions between Zanthexylum bungeanum Maxim and fake ones. Results:Length of cpDNA trnL-F of Zanthexylum bungeanum Maxim is 349bp with 62.1percent of AT sites. The difference is obvious between Zanthexylum bungeanum Maxim and fake ones in their cpDNA trnL-F regions though there's little diversity from their appearance of fruits together with physical characters. While this internal space has high alikeness among taxa classified by genus below, reaching full percent parallel. Conclusion:cpDNA trnL-F sequence is only suitable for the authentication of Zanthexylum bungeanum Maxim. from fake ones on the basis of Genus above. |
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| Keywords: | Zanthexylum bungeanum Maxim cpDNA trnL-F sequence Fake ones Authentication |
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