Lipid-polyethylene glycol interactions: I. Induction of fusion between liposomes |
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Authors: | L T Boni T P Stewart J L Alderfer S W Hui |
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Institution: | (1) Department of Biophysics, Roswell Park Memorial Institute, 14263 Buffalo, New York |
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Abstract: | Summary Fusion between unilamellar vesicles of both egg phosphatidylcholine and bovine phosphatidylserine was induced by polyethylene glycol. Aggregation and fusion events were monitored by electron microscopy and turbidity measurements. The threshold concentration of polyethylene glycol for aggregation and fusion is found to be independent of lipid concentration. Typically, aggregation of phosphatidylcholine vesicles starts at 2.5% (wt/wt) polyethylene glycol, but fusion is not significant until the polyethylene glycol concentration reaches 35%. Multilamellar vesicles were formed as a result of fusion.Abbreviations PEG
Polyethylene glycol
- IMP
Intramembranous particle
- PC
Phosphatidylcholine
- PS
Phosphatidylserine
- SUV
Small unilamellar vesicles
- MLV
Multilamellar vesicles
- DPPC
Dipalmitoyl phosphatidylcholine
- DSC
Differential scanning calorimetry |
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Keywords: | Fusion polyethylene glycol unilamellar vesicles aggregation freeze fracture turbidity |
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