Utilization of agro-industrial waste for xylanase production by Aspergillus foetidus MTCC 4898 under solid state fermentation and its application in saccharification |
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Affiliation: | 1. BRD School of Biosciences, Sardar Patel Maidan, Satellite Campus, P. Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat, India;2. Department of Statistics, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat, India;1. Institut national de la recherche scientifique, Centre—Eau Terre Environnement, 490, Rue de la Couronne, Québec, QC, Canada G1K 9A9;2. Centre de recherche industrielle du Québec (CRIQ), 333 Franquet, Québec, QC, Canada G1P 4C7;1. Avantha Centre for Industrial Research & Development, Paper Mill Campus, Yamuna Nagar 135001, Haryana, India;2. Department of Biotechnology, Kurukshetra University, Kurukshetra 136119, Haryana, India;1. Department of Biotechnology, University of North Bengal, Siliguri 734013, India;2. Dept of Microbiology, University of North Bengal, Siliguri 734013, India |
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Abstract: | Xylanase production by Aspergillus foetidus MTCC 4898 was carried out under solid state fermentation using wheat bran and anaerobically treated distillery spent wash. Response surface methodology involving Box–Behnken design was employed for optimizing xylanase production. The interactions among various fermentation parameters viz. moisture to substrate ratio, inoculum size, initial pH, effluent concentration and incubation time were investigated and modeled. The predicted xylanase activity under optimized parameters was 8200–8400 U/g and validated xylanase activity was 8450 U/g with very poor cellulase activity. Crude xylanase was used for enzymatic saccharification of agroresidues like wheat straw, rice straw and corncobs. Dilute NaOH and ammonia pretreatments were found to be beneficial for the efficient enzymatic hydrolysis of all the three substrates. Dilute NaOH pretreated wheat straw, rice straw and corncobs yielded 4, 4.2, 4.6 g/l reducing sugars, respectively whereas ammonia treated wheat straw, rice straw and corncobs yielded 4.9, 4.7, 4.6 g/l reducing sugars, respectively. The hydrolyzates were analysed by HPTLC. Xylose was found to be the major end product with traces of glucose in the enzymatic hydrolyzates of all the substrates. |
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