Adsorptive bioconversion of ethylene glycol to glycolic acid by Gluconobacter oxydans DSM 2003 |
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| Affiliation: | 1. State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China;2. College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China;1. Takasago Thermal Engineering Co. Ltd., Shinjyuku, Tokyo 160-0022, Japan;2. Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture & Technology, Koganei, Tokyo 184-8588, Japan;3. Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki 305-8687 Japan;4. Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan;5. College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-0880, Japan;1. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China;2. Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China;1. Technical University of Denmark, Department of Chemical and Biochemical Engineering, Lyngby, Denmark;2. SINTEF, Materials and Chemistry, Biotechnology and Nanomedicine, Trondheim, Norway;1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;2. Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan 450002, China;1. Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, People’s Republic of China;2. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People’s Republic of China;3. Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, People’s Republic of China;4. ECO Zhuoxin Energy-saving Technology (Shanghai) Company Limited, Shanghai 200000, People’s Republic of China;5. Nanjing Hydraulic Research Institute, Materials & Structural Engineering Department, Nanjing 210029, People’s Republic of China |
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| Abstract: | An integrated bioprocess for the production of glycolic acid from ethylene glycol with Gluconobacter oxydans DSM 2003 and in situ product removal were investigated. A slight substrate inhibition was observed as substrate concentration was above 20 g/l and the product inhibition was much stronger. Bioconversion of glycolic acid is an end-product-inhibited reaction. In order to increase the productivity of glycolic acid and reduce the end-product inhibition of bioconversion, an adsorptive bioconversion for glycolic acid production from ethylene glycol catalyzed by resting cells of G. oxydans DSM 2003, was developed by using anion exchange resin D315 as the adsorbent for selective removal of glycolic acid from the reaction mixture. This approach allowed the yield of glycolic acid to be increased to 93.2 g/l, compared to 74.5 g/l obtained from a conventional fed-batch mode. |
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