Comparison of oxygen mass transfer coefficient in simple and extractive fermentation systems |
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| Affiliation: | 1. Department of Biochemical and Pharmaceutical Technology, University of São Paulo, Av. Prof. Lineu Prestes, 580, Bloco 16, Cidade Universitária, 05508-000 São Paulo, SP, Brazil;2. Department of Chemical and Process Engineering, University of Genoa, Via Opera Pia 15, 16145 Genoa, Italy;3. Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n, 52171-900 Dois Irmãos, PE, Brazil;1. Department of Chemical and Biomolecular Engineering, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea;2. Department of Chemistry, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea;3. Research Institute of Biotechnology, CJ CheilJedang, 55, Gwanggyo-ro 42-gil, Yeongtong-gu, Suwon 16495, Republic of Korea;1. Departamento de Farmácia, Universidade Federal de Sergipe, São Cristóvão, SE, Brazil;2. Departamento de Bioquímica, Universidade Federal de Rio Grande do Sul, Porto Alegre, RS, Brazil;3. Departamento de Farmácia, Universidade Federal de Pernambuco, Recife, PE, Brazil;4. Departamento de Fisiologia, Universidade Federal de Sergipe, São Cristóvão, SE, Brazil;1. Graduate School, Khon Khon University, Khon Kaen 40002, Thailand;2. Department of Chemical and Biological Engineering, University of Saskatchewan, Saskatoon, SK S7N5A9, Canada;3. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Thailand;1. Irstea, 17 Avenue de Cucillé, CS 64427, 35044 Rennes Cedex, France;2. UMR Ingénierie Procédés Aliments, AgroParisTech, INRA, Université Paris-Saclay, Massy, France;3. Laboratoires Standa, 68 Rue Robert Kaskoreff, Caen 14000, France |
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| Abstract: | Aeration and agitation are important variables to ensure effective oxygen transfer rate during aerobic bioprocesses; therefore, the knowledge of the volumetric mass transfer coefficient (kLa) is required. In view of selecting the optimum oxygen requirements for extractive fermentation in aqueous two-phase system (ATPS), the kLa values in a typical ATPS medium were compared in this work with those in distilled water and in a simple fermentation medium, in the absence of biomass. Aeration and agitation were selected as the independent variables using a 22 full factorial design. Both variables showed statistically significant effects on kLa, and the highest values of this parameter in both media for simple fermentation (241 s−1) and extractive fermentation with ATPS (70.3 s−1) were observed at the highest levels of aeration (5 vvm) and agitation (1200 rpm). The kLa values were then used to establish mathematical correlations of this response as a function of the process variables. The exponents of the power number (N3D2) and superficial gas velocity (Vs) determined in distilled water (α = 0.39 and β = 0.47, respectively) were in reasonable agreement with the ones reported in the literature for several aqueous systems and close to those determined for a simple fermentation medium (α = 0.38 and β = 0.41). On the other hand, as expected by the increased viscosity in the presence of polyethylene glycol, their values were remarkably higher in a typical medium for extractive fermentation (α = 0.50 and β = 1.0). A reasonable agreement was found between the experimental data of kLa for the three selected systems and the values predicted by the theoretical models, under a wide range of operational conditions. |
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