In vivo and in vitro embryo production in goats |
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| Institution: | 1. Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Camino Cruz Del Sur, 2250, Montevideo, Uruguay;2. Programa de Posgrado, Facultad de Veterinaria, Universidad de La República, Av. Lasplaces, 1550, Montevideo, Uruguay;3. Unidad de Animales Transgénicos y de Experimentación, Institut Pasteur de Montevideo, Mataojo, 2020, Montevideo, Uruguay;1. Faculdade de Veterinária, Universidade Federal Fluminense, Rua Vital Brazil, 64, CEP 24320-340, Niterói, RJ, Brazil;2. Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de acesso Prof. Dr. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP, Brazil;3. Embrapa Caprinos e Ovinos, Estrada Sobral/Groaíras, km 04, CP 145, CEP 62010-970, Sobral, CE, Brazil;4. Embrapa Gado de Leite, Rua Eugênio do Nascimento, 610, CEP 36038-330, Dom Bosco, Juiz de Fora, MG, Brazil;1. Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran;2. Department of Animal Science, Agricultural Research Agency of Sardinia, 07100 Sassari, Italy;3. Stem Cell Biology and Regenerative Medicine Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran;1. Universitat Autònoma de Barcelona, Departament de Ciència Animal i dels Aliments, 08193, Bellaterra, Barcelona, Spain;2. OVATEC, S.L. Parc Tecnològic del Vallès, Edificio Centro de Empresas de Nuevas Tecnologías, Av. Parc Tecnològic, 3, 08290, Cerdanyola del Vallès, Barcelona, Spain;1. Universidade Federal de Lavras, Av. Doutor Sylvio Menicucci, 1001, Kennedy, CEP 37200-900, Lavras, MG, Brazil;2. Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, CEP 24230-340, Niterói, RJ, Brazil;3. Universidade Federal Rural do Rio de Janeiro, Rodovia BR 465, Km 07, s/n Zona Rural, CEP 23890-000, Seropédica, RJ, Brazil;4. Universidade Estadual Paulista “Júlio de Mesquita Filho”, Via de acesso Prof. Paulo Donato Castelane, s/n, Zona Rural, CEP 14884-900, Jaboticabal, SP, Brazil;5. Embrapa Caprinos e Ovinos, Estrada Sobral/Groaíras, km 4, CP D10, CEP 62011-000, Sobral, CE, Brazil;1. Yunnan Animal Science and Veterinary Institute, Jindian, Panlong County, Kunming City, Yunnan Province, China;2. Yunnan Provincial Meat Caprine Engineering Research Center, Jindian, Panlong County, Kunming City, Yunnan Province, China;3. Yunnan Provincial Engineering Laboratory of Animal Genetic Resource Conservation and Germplasm Enhancement, Jindian, Panlong County, Kunming City, Yunnan Province, China;4. College of Animal Science and Technology, China Agricultural University, Yuanmingyuan West Road, Haidian County, Beijing, China |
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| Abstract: | Assisted reproductive technologies (ART) such as artificial insemination (AI) and multiple ovulation and embryo transfer (MOET) have been used to increase reproductive efficiency and accelerate genetic gain. The principal limitations of MOET are due to variable female response to hormonal treatment, fertilization failures and premature regression of Corpora luteum. The in vitro production (IVP) of embryos offers the possibility of overcoming MOET limitations. The method of IVP of embryos involves three main steps: in vitro maturation of oocytes (IVM), in vitro fertilization of oocytes (IVF) with capacitated sperm and in vitro culture (IVC) of embryos up to blastocyst stage. Recovering oocytes from live selected females by laparoscopic ovum pick-up (LOPU) and breeding prepubertal females by juvenile in vitro embryo technology (JIVET) will allow a greater production of valuable goats. Also, IVP of goat embryos will provide an excellent source of embryos for basic research on development biology and for commercial applications of transgenic and cloning technologies. Different protocols of IVP of embryos have been used in goats. However oocyte quality is the main factor for embryos reaching blastocyst stage from IVM/IVF/IVC oocytes. One of the principal determinant factors in the results of blastocyst development is the age of the oocyte donor females. In goats, oocytes from prepubertal and adult females do not show differences in in vitro maturation and in vitro fertilization; however the percentage of oocytes reaching blastocyst stage ranges from 12 to 36% with oocytes from prepubertal and adult goats, respectively. |
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