Classification of lesions and comparison of immunohistochemical and acid fast staining in diagnosis of naturally occurring paratuberculosis in goats |
| |
| Institution: | 1. Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, 22 Bahman Blvd., Kerman, Iran;2. Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;3. Department of Immunology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;1. Department of Veterinary Medicine, University of Perugia, via San Costanzo 4, 06126 Perugia, Italy;2. DVM Practitioner, Grosseto, Italy;3. University of Pretoria, Faculty of Veterinary Science, Department of Paraclinical Sciences, Onderstepoort, South Africa;1. Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran;2. Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran;3. Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran;4. Department of Epidemiology and Biostatistics, Research Center for Emerging and Reemerging Diseases, Pasteur Institute of Iran, Tehran, Iran;1. Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;2. Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran;3. Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;1. Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;2. Department of Food hygiene and Public Health, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran;3. Cereal Health Research Center, Golestan University of Medical Sciences, Gorgan, Iran;4. Department of Public Health, Golestan University of Medical Sciences, Gorgan, Iran;1. Departamento de Zootecnia, Universidade Federal de Viçosa, 36571-000 Viçosa, MG, Brazil;2. Embrapa South Animal Husbandry & Sheep, 96401-970 Bagé, RS, Brazil;3. Embrapa Agriculture Informatics, 13083-886 Campinas, SP, Brazil;4. Embrapa Dairy Cattle, 36038-330 Juiz de Fora, MG, Brazil;5. Epamig, 38001-970, Uberaba, MG, Brazil |
| |
| Abstract: | Lesions associated with paratuberculosis and degrees of positivity by Ziehl-Neelsen (ZN) and Avidin–Biotin Complex peroxidase (ABC) techniques in 26 adult goats grossly or clinically suspected to paratuberculosis were studied. Of these, 17 (65.4%) had microscopic lesions associated with paratuberculosis which were classified into three categories. Diffuse multibacillary lesions (30.8%) characterized by a diffuse granulomatous enteritis with epithelioid macrophages infiltration as main inflammatory cells into lamina propria that arranged mosaic-like appearance, and filled with numerous acid fast bacilli in ZN and highly positive in ABC. Diffuse lymphocytic lesions (11.5%) composed of lymphocytes as main inflammatory infiltrate, with some epithelioid macrophages or giant cells containing few if any mycobacteria. Diffuse mixed lesions (23%) characterized by a diffuse granulomatous enteritis with infiltration of mixture of large numbers of lymphocytes and epithelioid macrophages with varying degrees of positivity in ZN and ABC. In all types of lesions, other lesions such as granulomatous lymphangitis and arteritis, lymphangiectasis, thrombi composed of degenerated epithelioid macrophages were observed. Caseous necrosis, calcification and fibrosis in mesenteric lymph nodes commonly, especially in diffuse lymphocytic form, were observed. In conclusion, the present study showed three categories of lesions in which diffuse multibacillary form was the commonest type. Both ABC and ZN methods can detect (ABC with more positivity) almost all the goats with diffuse multibacillary and mixed lesions; more cases with lymphocytic forms were positive by ABC than by ZN. ABC method seems to be an efficient diagnostic adjunct to conventional ZN staining for diagnosis of paratuberculosis in goats. The importance of sampling the ileum (especially ileocecal valve), jejunum and mesenteric lymph nodes to find microscopic lesions of paratuberculosis in goats is emphasized. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
