Comparative evaluation of recombinant BP26 protein for serological diagnosis of Brucella melitensis infection in goats |
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| Institution: | 1. Pediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran;3. Department of Microbiology, Tabriz University of Medical Sciences, Tabriz, Iran;4. Department of Biochemistry, Iran University of Medical Sciences, Tehran, Iran;1. LUNAM University, Oniris, UPSP 5304 of Animal Pathophysiology and Functional Pharmacology, Atlanpôle La Chantrerie, BP 40706, 44307 Nantes, France;2. LUNAM University, Oniris, INRA UMR U703, PanTHER, Atlanpôle La Chantrerie, BP 40706, 44307 Nantes, France;3. LUNAM University, INSERM, UMR 1087/CNRS 6291, Institut du Thorax, Nantes F44007 France;4. LUNAM University, Oniris, INSERM UMR_S 791, LIOAD, Atlanpôle La Chantrerie, BP 40706, 44307 Nantes, France;5. LUNAM University, Oniris, UPSP SSBR, Atlanpôle La Chantrerie, BP 40706, 44307 Nantes, France;1. PG and Research Department of Physics, Pachaiyappa’s College, Chennai 600030, TN, India;2. Research and Development, St. Peter’s Institute of Higher Education and Research, St. Peter’s University, Avadi, Chennai 600054, TN, India |
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| Abstract: | An enzyme-linked immunosorbent assay (ELISA) was developed for the serological diagnosis of caprine brucellosis with purified recombinant BP26 (outer membrane protein 28) of Brucella melitensis 16M produced in Escherichia coli. Brucella protein named CP28, BP26, or Omp28 has been identified as an immunodominant antigen in infected cattle, sheep, goats, and humans. The recombinant BP26 (rBP6) ELISA performed with serum samples (n = 1738) taken from organized farms and field goats from Northern India and tested in two different batches of 778 and 960 animals for brucellosis. Positive results were compared with the traditional serum agglutination test (SAT), complement fixation test (CFT) and dot-ELISA. The diagnostic sensitivity of rBP26 ELISA, SAT, CFT and dot-ELISA was 87.5%, 56.25%, 62.5% and 75% respectively. The specificity of the rBP26 ELISA, SAT, CFT and dot-ELISA was 90%, 75%, 80%, and 85% respectively. The results of rBP26 ELISA positive animals were further compared and evaluated by tissue PCR using B. melitensis BP26 gene as target. This resulted in 100% positive correlation between rBP26 ELISA and BP26 PCR. Thus, these results confirmed the importance of BP26 as a suitable antigen and rBP26 ELISA, if well standardized, proved to be a good screening test for the serological diagnosis of caprine brucellosis. |
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