Objective assessment of goat sperm head size by computer-assisted sperm morphometry analysis (ASMA) |
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| Institution: | 1. ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India;2. Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India;1. Department of Population Health, College of Veterinary Medicine, The University of Georgia, Athens, Georgia, USA;2. Department of Large Animal Medicine, College of Veterinary Medicine, The University of Georgia, Athens, Georgia, USA;1. Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran;3. Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan;1. Pferdezentrum Bad Saarow, Equine Reproduction Unit, Freie Universität Berlin, Bad Saarow, Germany;2. Clinic for Horses, Freie Universität Berlin, Berlin, Germany;1. Laboratorio de Genética Molecular y Zoonosis, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma Benito Juárez de Oaxaca, Mexico;2. Departamento de Secuenciación Genómica, Centro Nacional de Servicios de Constatación en Salud Animal, Morelos, Mexico;3. Laboratorio de Marcadores Moleculares, Departamento de Genética, Universidad de Córdoba, Spain;4. Laboratorio de Investigación en Reproducción Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma Benito Juárez de Oaxaca, Mexico;1. Departamento de Reproducción Animal, SGIT-INIA, Avda. Puerta de Hierro km 5.9, 28040 Madrid, Spain;2. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), SHIS QI 1, Lago Sul, Brasília DF, 71605-001, Brazil;3. Facultad de Veterinaria, Universidad Complutense de Madrid, Av. Puerta de Hierro, s/n, 28040 Madrid, Spain |
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| Abstract: | The aim of the present study was to identify different objective categories of sperm head size evaluation, using both morphometric and statistical analyses. For this purpose, semen samples (n = 16) were collected from 4 Florida male goats and assessed using a computer-assisted sperm morphometric analysis (ASMA) to obtain sperm head size measurements. The sperm heads were grouped into categories according to 25th and 75th percentiles (the lower and upper quartile, respectively) of their area values. Thereafter, a discriminant analysis was implemented on all the sperm morphometric parameters assessed, to obtain a classification matrix for sperm head size. Sperm heads by this method were classified into 3 categories depending on their size (small, medium and large), with a globally correct assignment of 95.5%. Moreover, significant differences (p < 0.001) were recorded between individual animals for all the sperm head morphometric parameters assessed. In conclusion, by using both statistical and morphometric analyses, it was possible to recognize the three categories of sperm head size. It is expected that research could be useful in defining the relationship between sperm head size measurements and actual fertility data. |
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