Effect of mechanical agitation on the production of cellulases by Trichoderma reesei RUT-C30 in a draft-tube airlift bioreactor |
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| Institution: | 1. Horticulture Section, School of Integrative Plant Science, Cornell University, Geneva, NY, 14456, USA;2. Department of Forest and Natural Resources Management, SUNY College of Environmental Science and Forestry, Syracuse, NY, 13210, USA;3. Forest Biomass Innovation Center, Michigan State University, Escanaba, MI, 49829, USA;4. Department of Plant Science, The Pennsylvania State University, University Park, PA, 16802-3504, USA;5. Department of Plant Science, University of Connecticut, Storrs, CT, 06269, USA;6. Department of Crop Sciences, University of Illinois, Urbana, IL, 61801, USA;1. IRISA–INRIA, France;2. Université de Rennes 1, France;3. ENS Cachan Bretagne, Université Européenne de Bretagne, Campus de Ker Lann, Avenue Robert Schuman, 35170 Bruz, France;4. Computer Science Department, Colorado State University, USA;5. Campus de Beaulieu, 35000 Rennes, France;1. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China;2. University of Chinese Academy Sciences, 19A, Yuquan Road, Beijing 100049, China;1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China;2. State Key Laboratory of Microbial Metabolism & School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China |
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| Abstract: | With the aim to produce cellulases and to study the effect of mechanical agitation, a 35 L draft-tube airlift bioreactor equipped with a mechanical impeller was developed and validated to grow Trichoderma reesei RUT-C30 in a cellulose culture medium with lactose and lactobionic acid as fed batch. Cultures carried out without mechanical agitation resulted in higher volumetric enzyme productivity (200 U L?1 h?1), filter paper activity (17 U mL?1), carboxymethyl cellulase activity (11.8 U mL?1) and soluble proteins (3.2 mg mL?1) when compared to those with agitation. Stereo and polarized light microscopy analyses reveal that mechanical agitation resulted in shorter mycelial hyphae and larger numbers of tips. |
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