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Revealing of allelic polymorphism in the populations of parthenogenetic lizards <Emphasis Type="Italic">Darevskia dahli</Emphasis> (Lacertidae) using locus-specific PCR
Authors:A G Davoyan  A V Aslanyan  F D Danielyan  I S Darevsky  I A Martirosyan
Institution:(1) Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia;(2) Department of Zoology, Yerevan State University, Yerevan, 375049, Armenia;(3) Institute of Zoology, Russian Academy of Sciences, St. Petersburg, 119034, Russia
Abstract:Locus-specific PCR was used to study the genetic polymorphism in three populations of parthenogenetic lizard species Darevskia dahli. The analysis was carried at the two (GATA) n -containing loci (Du215 and Du281) using the sample of 26 individuals. A total of eight Du215 and three Du281 allelic variants were detected. It was demonstrated that all the lizards examined were heterozygous at these loci. In 12 animals, unusual Du215 allelic variant was revealed, the origin of which was thought to be associated with different types of genomic rearrangements, or segmental duplication. The populations studied were substantially different relative to the levels of allelic polymorphism, which could be explained by different habitation conditions, leading to accumulation of mutations in noncoding genome regions.
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