多肽抗生素apidaecin基因在乳酸乳球菌中的融合表达

利用乳链菌肽(nisin)诱导表达系统，以泛素(ubiquitin)融合蛋白的形式在乳酸乳球菌(Lactococcus lactis)中表达了多肽抗生素apidaecin。利用TricineSDSPAGE和Western blotting均可在诱导后的宿主菌中检测到特异蛋白带。表达产物的最高产量可达宿主菌可溶性蛋白的7.2%左右。在体外用泛素特异性蛋白酶UBPI从融合蛋白中切除泛素后，产物具有明显的抗菌活性。

Fusion Expression of a Peptide Antibiotic-apidaecin Gene in Lactococcus lactis

The ubiquitin fusion of apidaecin was expressed in Lactococcus lactis ,using a novel nisin inducible expression system.After induction,a specific band could be detected in the extracts of the host strain by Tricine SDS PAGE and Western blotting. Production of the fusion was up to 7 2% of the total soluble protein of the host strain.While the fusion was cut by ubiquitin specific protease|UBP1,the product had distinct antibacterial activity.