Cloning of the protein D2 gene of Pseudomonas aeruginosa and its functional expression in the imipenem-resistant host |
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Authors: | H Yoneyama T Nakae |
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Institution: | Department of Cellular Information Sciences, Tokai University, School of Medicine, Isehara, Japan. |
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Abstract: | Protein D2 forms the water-filled pore across the outer membrane of Pseudomonas aeruginosa and allows the penetration of imipenem. We cloned the protein D2 gene by the antibody screening technique. When the imipenem-resistant mutant lacking protein D2 harbored the plasmid with the cloned D2 gene, the mutant overproduced protein D2 in the outer membrane. These transformants exhibited fully-restored imipenem susceptibility. The results prove unequivocally that protein D2 forms the imipenem-permeable pore in the P. aeruginosa outer membrane. |
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