Separation of prostaglandins E2 and F2α from their pulmonary metabolites by thin-layer chromatography |
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Authors: | J.Bryan Smith Melvin J. Silver Carol M. Ingerman |
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Abstract: | A simple thin-layer chromatographic system has been developed to separate PGE2 and PGF2α from 13,14-dihydro-PGF2α and other pulmonary metabolites of prostaglandins. The separation is achieved on Kieselgel-60 F-254 plates impregnated with both silver nitrate and boric acid. The system should be of use in the purification and tentative identification of primary prostaglandins. |
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