| 拉萨市藏族人群15个STR基因座多态性的研究 |
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| 引用本文： | 李宁,苏玉虹,席焕久,任甫,朱宝芹,温有峰. 拉萨市藏族人群15个STR基因座多态性的研究[J]. 遗传, 2006, 28(11): 1361-1364. DOI: 10.1360/yc-006-1361 |
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| 作者姓名： | 李宁 苏玉虹 席焕久 任甫 朱宝芹 温有峰 |
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| 作者单位： | 1. 辽宁省锦州医学院基础学院生物化学教研室, 锦州 121001; 2. 辽宁省锦州医学院辽宁省分子细胞生物学与新药开发重点实验室, ;锦州 121001; 3. 辽宁省锦州医学院人类学研究所, 锦州 121001 ; |
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| 基金项目： | 国家自然科学基金，辽宁省教育厅资助项目 |
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| 摘 要： | 利用多重PCR和五色荧光(6FAM、VIC、NED、PET、LIZ)自动化检测技术调查西藏自治区拉萨市藏族人群D8S1179、D21S11、D7S820、CSF1PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、VWA、TPOX、D18S51、D5S818、FGA共１5个STR基因座多态性分布, 获得了15个STR基因座的遗传学数据。结果显示: 15个STR基因座的基因型分布符合Hardy-Weinberg平衡。 15个STR基因座的个体鉴别力(DP)在0.7515~0.9599之间, 杂合度(H)在0.5576~0.8538之间, 多态信息含量(PIC)在0.5455~0.8458之间, 非父排除率(EPP)在0.3755~0.8520之间, 累积个体鉴别力为0.99999999, 累积非父排除率为0.999999997。15个STR基因座适合作为藏族人群的遗传标志用于人类学、遗传疾病连锁分析、法医学亲子鉴定和个体识别等研究领域。
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| 关 键 词： | 多态性 短串联重复序列 藏族 聚合酶链式反应 |
| 文章编号： | 0253-9772(2006)11-1361-04 |
| 收稿时间： | 2005-11-22 |
| 修稿时间： | 2006-03-03 |
Genetic Polymorphism of 15 STR Loci in a Lhasa Tibetan Population |
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| LI Ning,SU Yu-Hong,XI Huan-Jiu,REN Fu,ZHU Bao-Qin,WEN You-Feng. Genetic Polymorphism of 15 STR Loci in a Lhasa Tibetan Population[J]. Hereditas, 2006, 28(11): 1361-1364. DOI: 10.1360/yc-006-1361 |
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| Authors: | LI Ning SU Yu-Hong XI Huan-Jiu REN Fu ZHU Bao-Qin WEN You-Feng |
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| Affiliation: | 1. College of Basic Science, Jinzhou Medical College, Liaoning Province, Jinzhou 121001, China; 2. Key Laboratory of Molecular ;Cell Biology and New Drug Development of Liaoning Province, Jinzhou Medical College, Liaoning Province, Jinzhou 121001, China;3. Anthropology Laboratory, Jinzhou Medical College, Liaoning Province, Jinzhou 121001, China ; |
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| Abstract: | The polymorphism distributions of 15 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818 and FGA) were investigated in a Lhasa Tibetan population by multiplex PCR amplification using five fluorochromes (6FAM, VIC, NED, PET, LIZ). Gene frequency, discrimination power (DP), heterozygosity (H), polymorphism information content (PIC) and probability of paternity exclusion (EPP) were calculated, and all loci were tested for Hardy-Weinberg equilibrium. Results indicate that the gene frequency of these 15 STR loci is in Hardy-Weinberg equilibrium. The DP is at 0.7515-0.9599, H is at 0.5576-0.8538, PIC is at 0.5455-0.8458, and EPP isat 0.3755-0.8520. Cumulative DP of the 15 STR is 0.99999999, and cumulative EPP is 0.999999997. Therefore, these 15 STR loci can be used as genetic markers of in Lhasa Tibetan populations in anthropological studies, linkage analysis of genetic diseases, individual identification and paternity testing in forensic medicine. |
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| Keywords: | short tandem repeat (STR) polymorphism tibetan polymerase chain reaction (PCR) |
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