干酪乳杆菌LC2W细胞壁组分对RAW264．7巨噬细胞吞噬活性的影响

目的探讨干酪乳杆菌LC2W细胞壁组分体外对小鼠巨噬细胞功能的影响。方法以培养液单纯培养小鼠巨噬细胞系RAW264．7细胞作为对照，研究干酪乳杆菌LC2W细胞壁主要组分磷壁酸和肽聚糖对RAW264．7细胞乳酸脱氢酶（LDH）活性、吞噬中性红和致病菌能力的影响。结果不同浓度磷壁酸和肽聚糖对小鼠巨噬细胞RAW264．7细胞LDH活性、吞噬中性红能力有明显增强作用，并呈一定的剂量效应。在相同质量浓度时，2种细胞壁组分刺激RAW264．7细胞吞噬中性红能力差异无显著性，但磷壁酸对巨噬细胞RAW264．7细胞内LDH活性的增强作用高于肽聚糖。在受到浓度为50μg/ml的磷壁酸和肽聚糖刺激后，磷壁酸和肽聚糖均能显著增强RAW264．7对致病性大肠埃希菌和肠炎沙门菌的吞噬作用（P〈0．01）。经过刺激的巨噬细胞与致病菌共孵育1h后，其吞噬能力达到最大值。结论干酪乳杆菌LC2W细胞壁主要组分磷壁酸和肽聚糖可以增强小鼠巨噬细胞RAW264．7细胞内LDH活性及吞噬能力，并具有剂量效应。

The effect of cell wall components of L. casei LC2W on phagocytic activity of murine macrophage cells

Objective To investigate the effect of cell wall components of L. casei LC2W on phagocytic activity of murine macrot,hage cells in vitro. Methods Enhancement of the activity of Lactate Dehydrogenase （ LDH）, phagocytic activity to neutral red as well as cnteropathogeic pathogens of murine macrophage RAW264.7 cells challenged with different levels of teichoic acid （TA） or peptidoglycan （PG）, two predominant components of the cell wall of the cell L. casei LC2W, were compared with those macrophage cells challenged without TA or PG. Results The activity of LDH,phagocytic activity to neutral red as well as enteropathogeic pathogens were significantly enhanced,in a dosage-dependent manner,in murine macrophage RAW264.7 cells challenged with different levels of TA or PG. No obvious difference in phagocytic activity to neutral red existed in RAW264.7 cells challenged with LactobacillusTA and those cells challenged with PG. On the contrary,TA showed significant higher ability in enhancing LDH activity than PG. Simultaneously,TA and PG demonstrated similar ability in enhancing phagocytic activity of RAW264.7 cells to enteropathogenic microbes, when the latter were challenged with either TA or PG at the concentration of 50ug per milliliter. The maximized phagocytosis was reached after the challenged RAW264.7 cells were incubated with either Escherichia coli EPEC AS 1.72 or Salmonella enteritis AS 50041. Conclusion TA and PG of the cell wall of L. casei LC2W could significantly enhance the activity of LDH, phagocytic activity to neutral red as well as enteropathogeic pathogens, in a dosage-dependent manner, in murine macrophage RAW264.7 cells.