Primary culture and maintenance of steroid-secreting human placental monolayers |
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Authors: | Pamela W Roy Gail E Ryan Edwin D Bransome |
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Institution: | (1) Departments of Medicine and Endocrinology and the Center for Population Studies, Medical College of Georgia, 30902 Augusta, Georgia |
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Abstract: | Summary A simple method is described for primary culture and for maintenance of hormone-producing cells from normal human placenta.
A consistent yield of cells was obtained and an average survival of 3 to 4 months in culture using 1 mm3 explants from the most vascular area of the placentas. These explants were placed in a variety of culture media in 30 ml
flasks and incubated at 37°C in an atmosphere of 5% CO2 and 95% air. The best yields in terms of cell growth were observed with Eagle’s MEM (minimum essential medium) with supplements
of horse serum and fetal calf serum or human cord serum. (Ham’s F-10 with supplement of horse serum and fetal calf serum supports
growth for the longest period and media containing human cord serum had the best yield of steroids.)
Presented in part at the 25th meeting of the Tissue Culture Association, June 5, 1974, Miami Beach, Florida. Supported by
a contract from the Agency for International Development (2491/csd) and a grant from the National Institutes of Health (CA-12455). |
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Keywords: | placenta primary culture media steroid production |
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