The Use of Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Cacao Swollen Shoot Virus (CSSV) in Theobroma cacao |
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| Authors: | W Sagemann Dr H L Paul Dr D Adomako Dr G K Owusu |
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| Institution: | Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Viruskrankheiten der Pflanzen, Braunschweig, F. R. of Germany, Cocoa Research Institute of Ghana (C.R.I.G.) Tafo, Ghana |
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| Abstract: | Cacao swollen shoot virus (CSSV) was readily detected in different parts of Theobroma cacao using the ELISA technique. Different plant tissues contained varying amounts of CSSV; highest concentrations were found in leaf lamina. Methods to preserve the serological activity of CSSV were evaluated, and best results obtained with samples stored in a buffer or freeze-dried. |
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