Structure,expression, chromosomal location and product of the gene encoding ADH1 inPetunia |
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| Authors: | Robert Gregerson Michael McLean Marcel Beld Anton G M Gerats Judith Strommer |
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| Institution: | (1) Department of Genetics, University of Georgia, 30602 Athens, GA, USA;(2) Department of Molecular Biology and Genetics, University of Guelph, NIG 2W1 Guelph, Ontario, Canada;(3) Department of Genetics, Free University, De Boelelaan 1087, 1081 HV Amsterdam, Netherlands;(4) Department of Horticultural Science, University of Guelph, NIG 2W1 Guelph, Ontario, Canada;(5) Department of Molecular Biology and Genetics, University of Guelph, NIG 2W1 Guelph, Ontario, Canada |
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| Abstract: | A genomic clone for an alcohol dehydrogenase (Adh) gene has been isolated fromPetunia hybrida cv. V30 by screening aPetunia genomic library with a maizeAdh1 probe. A combination of RFLP and allozyme segregation data failed to demonstrate which of twoAdh loci, both of which map to chromosome 4, was the source of the cloned gene. The product of the cloned genes has been identified unequivocally by a transient expression assay inPetunia protoplasts. We have designated this genePetunia Adh1. The expression of this gene is tightly regulated in the developing anther, where its gene product is the predominant ADH isozyme. It is anaerobically inducible in roots, stems and leaves of seedlings. The induction of enzyme activity is correlated with induction ofAdh1 mRNA. |
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| Keywords: | alcohol dehydrogenase anaerobiosis chromosome mapping gene expression Petunia hybrida plant development |
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