Anti-ice nucleation activity in xylem extracts from trees that contain deep supercooling xylem parenchyma cells |
| |
| Institution: | 1. School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China;2. Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, South China University of Technology, Guangzhou 510640, China;3. College of Science and Engineering, Jinan University, Guangzhou 510632, China;1. Department of Chemistry, Faculty of Arts and Sciences, Niğde University, 51100 Nigde, Turkey;2. Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey;1. Service d’anesthésie et de réanimation, hôpital de la Conception, Assistance publique–Hôpitaux de Marseille, 147, boulevard Baille, 13385 Marseille cedex 5, France;2. Aix Marseille université, 13005 Marseille, France;1. Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 120-750, Republic of Korea;2. School of Food and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea;3. Institute of Agricultural Science & Technology, Kyungpook National University, Daegu 41566, Republic of Korea;4. Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, CA 92093-0204, USA;1. Institute for Global Food Security, School of Biological Sciences, Queen''s University Belfast, 18-30 Malone Road, Belfast, Co. Antrim, Northern Ireland BT9 5BN, United Kingdom;2. Department of Animal Pathology, University of Turin, Via L. Da Vinci 44, 10095 Grugliasco (TO), Italy;1. Computational Chemistry Research Group, Ton Duc Thang University, Ho Chi Minh City, Viet Nam;2. Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Viet Nam;3. Institute for Computational Science and Technology, Ho Chi Minh City, Viet Nam;4. Department of Chemistry, KU Leuven, Celestijnenlaan 200F, B-3001 Leuven, Belgium |
| |
| Abstract: | Boreal hardwood species, including Japanese white birch (Betula platyphylla Sukat. var. japonica Hara), Japanese chestnut (Castanea crenata Sieb. et Zucc.), katsura tree (Cercidiphyllum japonicum Sieb. et Zucc.), Siebold’s beech (Fagus crenata Blume), mulberry (Morus bombycis Koidz.), and Japanese rowan (Sorbus commixta Hedl.), had xylem parenchyma cells (XPCs) that adapt to subfreezing temperatures by deep supercooling. Crude extracts from xylem in all these trees were found to have anti-ice nucleation activity that promoted supercooling capability of water as measured by a droplet freezing assay. The magnitude of increase in supercooling capability of water droplets in the presence of ice-nucleation bacteria, Erwinia ananas, was higher in the ranges from 0.1 to 1.7 °C on addition of crude xylem extracts than freezing temperature of water droplets on addition of glucose in the same concentration (100 mosmol/kg). Crude xylem extracts from C. japonicum provided the highest supercooling capability of water droplets. Our additional examination showed that crude xylem extracts from C. japonicum exhibited anti-ice nucleation activity toward water droplets containing a variety of heterogeneous ice nucleators, including ice-nucleation bacteria, not only E. ananas but also Pseudomonas syringae (NBRC3310) or Xanthomonas campestris, silver iodide or airborne impurities. However, crude xylem extracts from C. japonicum did not affect homogeneous ice nucleation temperature as analyzed by emulsified micro-water droplets. The possible role of such anti-ice nucleation activity in crude xylem extracts in deep supercooling of XPCs is discussed. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
