Refolding of single-chain Fv by use of an antigen-coupled column |
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| Affiliation: | 1. Department of Material Chemistry, Kyoto Institute of Technology, 1 Hashigami-Cho, Matsugasaki, Sakyo-ku, Kyoto, Other, 606-8585, Japan;2. Emaus Kyoto, Inc., 26 Saiinnishida-Cho, Ukyo, Kyoto 615-0055, Japan;3. National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan;4. Kyoto Research Laboratories, YMC Co., Ltd., 59 Yonnotsubo-Cho Iwakuraminami, Sakyo, Kyoto 606-0033, Japan |
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| Abstract: | A single-chain variable fragment (scFv) antibody against bisphenol A was refolded using an antigen (bisphenol A)-coupled column. The refolding efficiency was compared with that used in dialysis. The refolding efficiency of the antigen-coupled column was about 50–60%, which was much higher than with dialysis, due to a decrease in the concentration of the refolding molecules and to the suppression of the aggregate formation. |
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